Carvacrol is present in the essential oil of Origanum vulgare (oregano), oil of thyme, oil obtained from pepperwort, and wild bergamot. The essential oil of Thyme subspecies contains between 5% and 75% of carvacrol, while Satureja (savory) subspecies have a content between 1% and 45%. Origanum majorana (marjoram) and Dittany of Crete are rich in carvacrol, 50% resp. 60-80%. It is also found in tequila.

Oregano is used for respiratory tract disorders such as coughs, asthma, croup, and bronchitis. It is also used for gastrointestinal (GI) disorders such as heartburn and bloating. Other uses include treating menstrual cramps, rheumatoid arthritis, urinary tract disorders including urinary tract infections (UTIs), headaches, and heart conditions.

The oil of oregano is taken by mouth for intestinal parasites, allergies, sinus pain, arthritis, cold and flu, swine flu, earaches, and fatigue. It is applied to the skin for skin conditions including acne, athlete's foot, oily skin, dandruff, canker sores, warts, ringworm, rosacea, and psoriasis; as well as for insect and spider bites, gum disease, toothaches, muscle pain, and varicose veins. Oregano oil is also used topically as an insect repellent.

In foods and beverages, oregano is used as a culinary spice and a food preservative.